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46-509 (0.5ug/ml) staining of U937 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-509 (2.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-509 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-509 (0.5ug/ml) staining of U937 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TRAF1 Antibody

CATALOG NUMBER: 46-509

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-KLQSPKHAYVKDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al , Am J Respir Cell Mol Biol. 2000 Feb;22(2):150-6. PMID: 10657935). Recommended concentration: 0.3-1.0ug/ml.Immunohistochemistry:Paraffin embedded Human Thymus and Small Intestine. Recommended concentration: 2.5ug/ml.
Positive Control 1:
Cat. No. 1215 - U937 Whole Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRAF1
Additional Names:
TRAF1, TNF receptor-associated factor 1 , EBI6, MGC:10353 , Epstein-Bar virus-induced protein 6
Protein Accession Number:
NP_005649.1
Protein Gi Number:
5032193
Ncbi Gene Id Number:
7185
Background Reference 1:
Su X, Li S, Meng M, Qian W, Xie W, Chen D, Zhai Z, Shu HB. TNF receptor-associated factor-1 (TRAF1) negatively regulates Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF)-mediated signaling. Eur J Immunol. 2006 Jan;36(1):199-206.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-509

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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