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46-627 (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-627 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-627 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-627 (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Arylsulfatase A Antibody

CATALOG NUMBER: 46-627

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-YDLSKDPGENYN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 53.6kDa according to human NP_000478.2 and 53.7kDa according to Mouse NP_033843.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARSA
Additional Names:
ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase
Protein Accession Number:
NP_000478.2
Protein Gi Number:
6005990
Ncbi Gene Id Number:
410
Background Reference 1:
Sevin C, Benraiss A, Van Dam D, Bonnin D, Nagels G, Verot L, Laurendeau I, Vidaud M, Gieselmann V, Vanier M, De Deyn PP, Aubourg P, Cartier N. Intracerebral adeno-associated virus-mediated gene transfer in rapidly progressive forms of metachromatic leukodystrophy. Hum Mol Genet. 2006 Jan 1;15(1):53-64. Epub 2005 Nov 25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-627

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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