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46-630 (0.03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-630 (0.03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AVPR1A Antibody

CATALOG NUMBER: 46-630

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CHPLKTLQQPARRSR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Frontal cortex) lysates and in Rat Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 46.8kDa according to NP_000697.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Phalipou et al, J Biol Chem. 1997 Oct 17;272(42):26536-44; PMID: 9334232). Recommended concentration: 0.03-0.1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AVPR1A
Additional Names:
AVPR1A, arginine vasopressin receptor 1A, AVPR1 , SCCL vasopressin subtype 1a receptor, V1-vascular vasopressin receptor AVPR1A, V1a vasopressin receptor, antidiuretic hormone receptor 1A, vascular/hepatic-type arginine vasopressin receptor
Protein Accession Number:
NP_000697.1
Protein Gi Number:
4502331
Ncbi Gene Id Number:
552
Background Reference 1:
Yirmiya N, Rosenberg C, Levi S, Salomon S, Shulman C, Nemanov L, Dina C, Ebstein RP. Association between the arginine vasopressin 1a receptor (AVPR1a) gene and autism in a family-based study: mediation by socialization skills. Mol Psychiatry. 2006 May;11(5):488-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-630

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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