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46-632 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing BDH2 and probed with 46-632 (mock transfection in first lane).
46-632 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-632 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BDH2 Antibody

CATALOG NUMBER: 46-632

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-TKKKQIDQFANE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BDH2
Additional Names:
BDH2, DHRS6, 3-hydroxybutyrate dehydrogenase, type 2, EFA6R, FLJ13261, PRO20933, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, oxidoreductase UCPA
Protein Accession Number:
NP_064524.3
Protein Gi Number:
66933014
Ncbi Gene Id Number:
56898
Background Reference 1:
Guo K, Lukacik P, Papagrigoriou E, Meier M, Lee WH, Adamski J, Oppermann U. Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase. J Biol Chem. 2006 Apr 14;281(15):10291-7. Epub 2005 Dec 27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-632

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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