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46-634 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing BHMT and probed with 46-634 (mock transfection in first lane).
46-634 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-634 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-634 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BHMT Antibody

CATALOG NUMBER: 46-634

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-EQQLKELFEKQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BHMT
Additional Names:
BHMT, betaine-homocysteine methyltransferase
Protein Accession Number:
NP_001704.1
Protein Gi Number:
4502407
Ncbi Gene Id Number:
635
Background Reference 1:
Collinsova M, Strakova J, Jiracek J, Garrow TA. Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice. J Nutr. 2006 Jun;136(6):1493-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-634

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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