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46-638 (0.5ug/ml) staining of Jurkat (A), (0.3ug/ml) PBM (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-638 (2ug/ml) staining of Human Spleen (A) and Mouse Thymus (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-638 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
46-638 (0.5ug/ml) staining of Jurkat (A), (0.3ug/ml) PBM (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Carabin Antibody

CATALOG NUMBER: 46-638

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-PRRKPQTRGKTFH
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TBC1D10C
Additional Names:
TBC1D10C, carabin, TBC1 domain family, member 10C, FLJ00332, MGC46488
Protein Accession Number:
NP_940919.1
Protein Gi Number:
38348348
Ncbi Gene Id Number:
374403
Background Reference 1:
Pan F, Sun L, Kardian DB, Whartenby KA, Pardoll DM, Liu JO. Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin. Nature. 2007 Jan 25;445(7126):433-6. Epub 2007 Jan 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-638

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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