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46-644 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. Acti
46-644 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-644 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. Acti

CLEC16A Antibody

CATALOG NUMBER: 46-644

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SLENQDKGGERP
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to Rat XP_213209.4.Immunofluorescence: Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLEC16A
Additional Names:
CLEC16A, C-type lectin domain family 16, member A, Gop-1, KIAA0350, MGC111457
Protein Accession Number:
NP_056041.1,NP_001230332.1
Protein Gi Number:
54792782
Ncbi Gene Id Number:
23274
Background Reference 1:
Hakonarson H, Grant SF, Bradfield JP, Marchand L, Kim CE, Glessner JT, Grabs R, Casalunovo T, Taback SP, Frackelton EC, Lawson ML, Robinson LJ, Skraban R, Lu Y, Chiavacci RM, Stanley CA, Kirsch SE, Rappaport EF, Orange JS, Monos DS, Devoto M, Qu HQ, Poly A genome-wide association study identifies KIAA0350 as a type 1 diabetes gene. Nature. 2007 Aug 2;448(7153):591-4. Epub 2007 Jul 15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-644

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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