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ANK1 Antibody

CATALOG NUMBER: 46-725

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QIVKRASLKRGKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANK1
Additional Names:
ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
Protein Accession Number:
NP_000028.3,NP_065208.2,NP_065209.2,NP_065210.2,NP_065211.2,NP_065213.2
Protein Gi Number:
70780357
Ncbi Gene Id Number:
286
Background Reference 1:
Dubreuil RR. Functional links between membrane transport and the spectrin cytoskeleton. J Membr Biol. 2006;211(3):151-61. Epub 2006 Nov 7. Review.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-725

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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