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46-748 (2ug/ml) staining of paraffin embedded Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-748 (2ug/ml) staining of paraffin embedded Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

ApoA-IV Antibody

CATALOG NUMBER: 46-748

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-QETERVKEEIKKELED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:In paraffin embedded Mouse Colon shows staining of cells between the villi, consistent with the observations with the related EB07581. Recommended concentration: 2-4ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Apoa4
Additional Names:
Apoa4, apolipoprotein A-IV, Apoa-4
Protein Accession Number:
NP_031494.2
Protein Gi Number:
110347473
Ncbi Gene Id Number:
11808(mouse);
Background Reference 1:
Orsó E, Moehle C, Boettcher A, Szakszon K, Werner T, Langmann T, Liebisch G, Buechler C, Ritter M, Kronenberg F, Dieplinger H, Bornstein SR, Stremmel W, Schmitz G. The satiety factor apolipoprotein A-IV modulates intestinal epithelial permeability through its interaction with alpha-catenin: implications for inflammatory bowel diseases. Horm Metab Res. 2007 Aug;39(8):601-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-748

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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