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ABCB8 Antibody

CATALOG NUMBER: 46-793

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-KKPEGPRSHQHK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 22kDa band in lysates of cell lines HeLa and Jurkat after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_009119.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCB8
Additional Names:
ABCB8, ATP-binding cassette, sub-family B (MDR/TAP), member 8, EST328128, M-ABC1, MABC1, ATP-binding cassette, sub-family B, member 8, mitochondrial ABC protein
Protein Accession Number:
NP_009119.2
Protein Gi Number:
156105685
Ncbi Gene Id Number:
11194
Background Reference 1:
Hogue DL, Liu L, Ling V. Identification and characterization of a mammalian mitochondrial ATP-binding cassette membrane protein. J Mol Biol. 1999 Jan 8;285(1):379-89

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-793

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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