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46-794 (0.05ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-794 (0.05ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABCC1 Antibody

CATALOG NUMBER: 46-794

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HQSDLKVDENQKAYY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in lysates of cell line A549 (calculated MW of 159kDa according to NP_063954.2). Recommended concentration:0.05-0.2ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_004987.2; NP_063915.2; NP_063953.2; NP_063954.2; NP_063955.2).
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCC1
Additional Names:
ABCC1, ATP-binding cassette, sub-family C (CFTR/MRP), member 1, ABC29, ABCC, DKFZp686N04233, DKFZp781G125, GS-X, MRP, MRP1, ATP-binding cassette, sub-family C, member 1, LTC4 transporter, leukotriene C(4) transporter, multidrug resistance protein, multiple drug resistance protein 1, multiple drug resistance-associated protein
Protein Accession Number:
NP_004987.2,NP_063915.2,NP_063953.2,NP_063954.2,NP_063955.2
Protein Gi Number:
134142337
Ncbi Gene Id Number:
4363
Background Reference 1:
Bobrowska-Hägerstrand M, Wróbel A, Rychlik B, Ohman I, Hägerstrand H. Flow cytometric monitoring of multidrug drug resistance protein 1 (MRP1/ABCC1) -mediated transport of 2', 7'-bis-(3-carboxypropyl)-5-(and-6)- carboxyfluorescein (BCPCF) into human erythrocyte membrane inside-out vesicles. Mol Membr Biol. 2007 Sep-Dec;24(5-6):485-95.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-794

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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