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46-807 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
46-807 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-807 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Apolipoprotein L4 Antibody

CATALOG NUMBER: 46-807

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ENLNELTHIHQSLK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APOL4
Additional Names:
apolipoprotein L4, APOL4, apolipoprotein L, 4, APOL-IV, APOLIV, OTTHUMP00000028937, apolipoprotein L-IV
Protein Accession Number:
NP_085146.2,NP_663693.1
Protein Gi Number:
19923626
Ncbi Gene Id Number:
80832
Background Reference 1:
Monajemi H, Fontijn RD, Pannekoek H, Horrevoets AJ. The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue. Genomics. 2002 Apr;79(4):539-46

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-807

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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