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46-810 (0.3ug/ml) staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
46-810 (0.3ug/ml) staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Arylsulfatase D Antibody

CATALOG NUMBER: 46-810

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DASNGYRALQWNA
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2)
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARSD
Additional Names:
arylsulfatase D, ARSD
Protein Accession Number:
NP_001660.2,NP_033667.2
Protein Gi Number:
71852584
Ncbi Gene Id Number:
414
Background Reference 1:
Lin YF, Yang J, Rosen BP. ArsD: an As(III) metallochaperone for the ArsAB As(III)-translocating ATPase. J Bioenerg Biomembr. 2007 Oct 23;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-810

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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