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46-875 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-875 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Neurotrophin 5 Antibody

CATALOG NUMBER: 46-875

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: FETRCKADNAEEG
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.4kDa according to NP_006170.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ghinelli et al, Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3352-7; PMID: 12882781). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NTF5
Additional Names:
NT4, neurotrophin 5 (neurotrophin 4/5) , NTF5, NT-4/5, NT5, NTF4, neurotrophic factor 4, neurotrophic factor 5, neurotrophin 4, neurotrophin 5
Protein Accession Number:
NP_006170.1
Protein Gi Number:
5453808
Ncbi Gene Id Number:
4909
Background Reference 1:
Nalbandian A, Pang AL, Rennert OM, Chan WY, Ravindranath N, Djakiew D. A novel function of differentiation revealed by cDNA microarray profiling of p75NTR-regulated gene expression. Differentiation. 2005 Oct;73(8):385-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-875

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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