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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-352, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-719. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-719, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.5 ng
<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-720. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-720, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng
<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-352, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [NP642]

CATALOG NUMBER: 10-352

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Recombinant SARS-CoV-2 nucleocapsid protein (full-length).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Purification:
The antibody was expressed in HEK293F cells, and subsequently obtained from cellsupernatant by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG, kappa
physical-state:
Liquid
Buffer:
PBS pH7.2
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
N
Additional Names:
SARS-CoV-2 (COVID-19) Nucleocapsid antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
Protein Accession Number:
QHD43423
Protein Gi Number:
1820552696
Ncbi Gene Id Number:
43740575
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10-352

List Size:
0.1 mg

List Price:

$498.00

Datasheet
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