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IHC testing of human melanoma stained with Melan-A antibody (A103). Note cytoplasmic staining of cells.
SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone A103). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human melanoma stained with Melan-A antibody (A103). Note cytoplasmic staining of cells.

Melan-A Antibody [A103]

CATALOG NUMBER: 33-132

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Recombinant human protein was used as the immunogen for the Melan-A antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1.0 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Due to differences in protocols and secondary antibody used, the Melan-A antibody may require titration for optimal performance.

1. FFPE staining is enhanced by boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MLANA
Additional Names:
MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
Ncbi Gene Id Number:
2315
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Antibody A103 is specific for Melan-A (likely an abbreviation for ‘melanocyte antigen’), also called MART-1, a unique antigen on melanoma cells recognized by CTLs (cytotoxic T lymphocytes), making it useful as a melanocyte differentiation marker. It is commonly used to identify metastatic tumors of melanocytic origin, as well as perivascular epithelioid cell tumors. Unlike another commonly used Melan-A / MART-1 antibody, M2-7C10, A103 also stains steroid hormone producing cells, making it valuable in identifying adrenocortical carcinomas.

Note: this protein was identified in 1994 by two different labs, one naming it MART-1 (melanoma antigen recognized by T-cells) and the other Melan-A. Both names are commonly used in literature, but clone A103 antibody is generally referred to as being anti-Melan-A.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-132

List Size:
100 ug

List Price:

$626.00

Datasheet
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