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Western blot testing of human HeLa cell lysate with PAX7 antibody (clone PAX7/497). Expected molecular weight: 55-57 kDa.
Western blot testing of human HeLa cell lysate with PAX7 antibody (clone PAX7/497). Expected molecular weight: 55-57 kDa.

PAX7 Antibody [PAX7/497]

CATALOG NUMBER: 33-184

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Rat, Zebrafish
Immunogen:
Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PAX7
Additional Names:
Paired box protein Pax-7, HuP1, PAX7, HUP1
Ncbi Gene Id Number:
5081
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
The PAX gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the PAX family of proteins have been linked to disease and cancer in humans. PAX7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that PAX7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding PAX7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-184

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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