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FACS testing of K562 cells with Alexa Fluor 488 conjugated Transferrin receptor antibody (green) and isotype control (gray).
SDS-PAGE Analysis of Purified, BSA-Free Transferrin Receptor Antibody (clone 66IG10). Confirmation of Integrity and Purity of the Antibody.
FACS testing of K562 cells with Alexa Fluor 488 conjugated Transferrin receptor antibody (green) and isotype control (gray).

Transferrin Receptor Antibody [66IG10]

CATALOG NUMBER: 33-208

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for this Transferrin receptor antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Transferrin receptor antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TFRC
Additional Names:
Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, CD71, Transferrin receptor protein 1, serum form, sTfR, TFRC
Ncbi Gene Id Number:
7037
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a ~90-95kDa protein which is identified as cell surface Transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. The antibody is highly specific to transferrin receptor and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of transferrin receptor. Its ligand is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-208

List Size:
100 ug

List Price:

$626.00

Datasheet
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