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IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (Hyb-8). Note cell membrane & cytoplasmic staining.
SDS-PAGE Analysis of Purified, BSA-Free Biotin Antibody (clone Hyb-8). Confirmation of Integrity and Purity of the Antibody.
IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (Hyb-8). Note cell membrane & cytoplasmic staining.

Biotin Antibody [Hyb-8]

CATALOG NUMBER: 33-236

Clonality:
Monoclonal
Tested Applications:
CM, Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
All Species
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
All Species
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Conjugate:
Unconjugated
Tested Applications:
CM, Flow, IF, IHC, WB
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
WB: 2-5 ug/ml
Confocal Microscopy

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-236

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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