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Formalin-fixed, paraffin-embedded human angiosarcoma stained with SM-MHC antibody (SPM201).
Formalin-fixed, paraffin-embedded human angiosarcoma stained with SM-MHC antibody (SPM201).

SM-MHC Antibody [SPM201]

CATALOG NUMBER: 33-449

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Bovine, Cat, Chicken, Dog, Guinea Pig, Human, Pig, Rabbit, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Cat, Chicken, Dog, Guinea Pig, Human, Pig, Rabbit, Rat
Immunogen:
Human uterus extract was used as the immunogen for the SM-MHC antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MYH11
Ncbi Gene Id Number:
4629
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-449

List Size:
100 ug

List Price:

$626.00

Datasheet
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