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SDS-PAGE Analysis of Purified, BSA-Free TLR2 Antibody (clone TLR2/221). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free TLR2 Antibody (clone TLR2/221). Confirmation of Integrity and Purity of the Antibody.

TLR2 Antibody [TLR2/221]

CATALOG NUMBER: 33-471

Clonality:
Monoclonal
Tested Applications:
Flow, Functional Assays, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Dog, Human, Monkey
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Dog, Human, Monkey
Immunogen:
Recombinant human protein was used as the immunogen for the TLR2 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, Functional Assays, IF, IHC-P, WB
Application Note:
Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the TLR2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TLR2
Additional Names:
TLR2 Antibody: TIL4, CD282, TIL4, Toll-like receptor 2
Ncbi Gene Id Number:
7097
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb reacts with human Toll-like receptor 2 (TLR2). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-471

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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