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Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).
Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).
Formalin-fixed, paraffin-embedded rat colon stained with Nuclear marker antibody (NM106).
Formalin-fixed, paraffin-embedded rat lung stained with Nuclear marker antibody (NM106).
SDS-PAGE Analysis of Purified, BSA-Free Nuclear Marker Antibody (clone NM106). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear marker antibody (NM106).

Nuclear Marker Antibody [NM106]

CATALOG NUMBER: 33-545

Clonality:
Monoclonal
Tested Applications:
Flow, ICC, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
Flow, ICC, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunocytochemistry (Acetone-fixed): 0.25-0.5 ug/ml for 30 min at RT
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Nuclear marker antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificity:
Does not react with Pig
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-545

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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