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IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
IHC testing of FFPE human fallopian tube with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
IHC testing of FFPE human uterus with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free ASRGL1 Antibody (clone CRASH/1289). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

ASRGL1 Antibody [CRASH/1289]

CATALOG NUMBER: 33-605

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein was used as the immunogen for this ASRGL1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ASRGL1 antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ASRGL1
Additional Names:
ASRGL1, asparaginase like 1, ALP, ALP1, FLJ22316, asparaginase-like 1 protein, CRASH
Ncbi Gene Id Number:
80150
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, belongs to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-605

List Size:
100 ug

List Price:

$626.00

Datasheet
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