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IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Western blot testing of 1) a partial recombinant protein and 2) human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.
SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone C3e/1308). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

CD3e Antibody [C3e/1308]

CATALOG NUMBER: 33-654

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
WB: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Optimal dilution of the CD3e antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD3E
Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-654

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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