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IHC testing of FFPE human cervical carcinoma with SOX-2 antibody (clone SOX2/1791). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human lung carcinoma with SOX-2 antibody (clone SOX2/1791). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of 1) partial human protein and 2) NCCIT lysate with SOX-2 antibody (clone SOX2/1791). Predicted molecular weight ~34 kDa.
IHC testing of FFPE human cervical carcinoma with SOX-2 antibody (clone SOX2/1791). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SOX2 Antibody [SOX2/1791]

CATALOG NUMBER: 33-743

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 176-305 of the human protein were used as the immunogen for the SOX-2 antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ELISA: 2-4 ug/ml; order BSA-free formulation for coating
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the SOX-2 antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
SOX2
Additional Names:
SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2
Ncbi Gene Id Number:
6657
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-743

List Size:
100 ug

List Price:

$626.00

Datasheet
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