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IHC testing of FFPE human cervical tissue with Poloplanin antibody (clone PDPN/1433). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Podoplanin Antibody (clone PDPN/1433). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human cervical tissue with Poloplanin antibody (clone PDPN/1433). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Podoplanin Antibody [PDPN/1433]

CATALOG NUMBER: 33-758

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A human recombinant partial protein corresponding to amino acids 24-126 was used as the immunogen for the Podoplanin antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
WB: 0.5-1 ug/ml
IF: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Podoplanin antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PDPN
Additional Names:
Podoplanin, Aggrus, Glycoprotein 36, Gp36, PA226 antigen, T1-alpha, T1A, PDPN {ECO:0000312, EMBL:AAH146682}
Ncbi Gene Id Number:
10630
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Podoplanin (PDPN) is a muco-protein which localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-758

List Size:
100 ug

List Price:

$626.00

Datasheet
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