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IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1582). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HeLa and 2) HEK293 cell lysate with Spectrin beta III antibody (clone SPTBN2/1582). Predicted molecular weight ~246 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1582). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1582). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SPTBN2 Antibody [SPTBN2/1582]

CATALOG NUMBER: 33-760

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
WB: 0.5-1 ug/ml
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
SPTBN2
Ncbi Gene Id Number:
6712
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alphaII (fodrin alpha) and spectrin betaI (fodrin beta) are present in other somatic cells. SPTBN2/Spectrin betaIII is highly homologous to both spectrin betaI and spectrin betaII. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-760

List Size:
100 ug

List Price:

$626.00

Datasheet
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