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IHC testing of FFPE thyroid tissue stained with Thyroglobulin antibody (clone TG157-2).
IHC testing of FFPE thyroid tissue stained with Thyroglobulin antibody (clone TG157-2).

Thyroglobulin Antibody [TG157-2]

CATALOG NUMBER: 33-859

Clonality:
Monoclonal
Tested Applications:
Flow, IHC
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The immunogen for this Thyroglobulin antibody was human thyroid epithelial cells.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Thyroglobulin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, k
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TG
Additional Names:
TG, AITD3, TDH3, Thyroglobulin, TGN
Ncbi Gene Id Number:
7038
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-859

List Size:
100 ug

List Price:

$626.00

Datasheet
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