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IHC testing of FFPE human breast carcinoma with Progesterone receptor antibody (clone PGSR17).
IHC testing of FFPE human breast carcinoma with Progesterone receptor antibody (clone PGSR17).

Progesterone Receptor Antibody [PGSR17]

CATALOG NUMBER: 33-892

Clonality:
Monoclonal
Tested Applications:
IHC
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human Progesterone receptor protein was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone receptor antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PGR
Additional Names:
Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3
Ncbi Gene Id Number:
5241
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-892

List Size:
100 ug

List Price:

$626.00

Datasheet
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