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IHC testing of FFPE human thyroid tissue with TTF1 antibody (clone HBNK2-1)
IHC testing of FFPE human lung tissue with TTF1 antibody (clone HBNK2-1)
IHC testing of FFPE rat lung tissue with TTF1 antibody (clone HBNK2-1)
IHC testing of FFPE human thyroid tissue with TTF1 antibody (clone HBNK2-1)

TTF1 Antibody [HBNK2-1]

CATALOG NUMBER: 33-928

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein was used as the immunogen for the TTF1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the TTF1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
NKX2-1
Additional Names:
NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, TITF1, TTF-1, NKX2.1, Homeobox protein NK-2 homolog A
Ncbi Gene Id Number:
7080
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-928

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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