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IHC testing of FFPE human pancreas with NSE antibody (clone NSEL1-1).
Western blot testing of human HeLa cell lysate with NSE antibody (clone NSEL1-1). Predicted molecular weight ~47 kDa.
IHC testing of FFPE human pancreas with NSE antibody (clone NSEL1-1).

NSE Antibody [NSEL1-1]

CATALOG NUMBER: 34-031

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western blot: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10mM Citrate Buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ENO2
Additional Names:
ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
Ncbi Gene Id Number:
2026
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-031

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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