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IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Recombinant

Recombinant CD20 Antibody [RMC20-1]

CATALOG NUMBER: 34-059

Clonality:
Recombinant Monoclonal
Tested Applications:
IHC
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC
Application Note:
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein A affinity chromatography
Clonality:
Recombinant Monoclonal
Isotype:
IgG, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MS4A1
Additional Names:
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Ncbi Gene Id Number:
931
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-059

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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