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IHC testing of FFPE human angiosarcoma with recombinant Glycophorin A antibody (clone GYPA/1725R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human angiosarcoma with recombinant Glycophorin A antibody (clone GYPA/1725R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Recombinant

Recombinant Glycophorin A Antibody [GYPA/1725R]

CATALOG NUMBER: 34-089

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human GPA protein was used as the immunogen for the recombinant Glycophorin A antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein A affinity chromatography
Clonality:
Recombinant Monoclonal
Isotype:
IgG, k
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
GYPA
Additional Names:
GYPA, glycophorin A (includes MN blood group), HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, Mi.V glycoprotein (24 AA), erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin Er
Ncbi Gene Id Number:
2993
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-089

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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