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To detect hCTGF by sandwich ELISA, using 100 ul/well antibody solution, a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTGF, 38-105, as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of normal human kidney. The recommended concentration is 0.5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hCTGF by sandwich ELISA, using 100 ul/well antibody solution, a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTGF, 38-105, as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.

CTGF Antibody

CATALOG NUMBER: 38-101

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μg/mL), a concentration of 6.0 - 9.0 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect hCTGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.
Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
CTGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CTGF
Additional Names:
CCN2, NOV2, HCS24, IGFBP8, CCN2, Connective tissue growth factor, CCN family member 2, IBP-8
Protein Accession Number:
P29279
Protein Gi Number:
116241320
Ncbi Gene Id Number:
1490
User Note:
Centrifuge vial prior to opening.
Background:
CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-101

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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