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To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained PC-3 and CaCo-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm.
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

sTNF-receptor II Antibody

CATALOG NUMBER: 38-123

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 μg/mL) a concentration of 15.0 - 20.0 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect hsTNF-receptor II by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Sandwich
To detect hsTNF-receptor II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human sTNF-receptor II as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Western Blot:
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
sTNF-receptor II antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNFRSF1B
Additional Names:
p75, TBPII, TNFBR, TNFR2, CD120b, TNFR1B, TNFR80, TNF-R75, p75TNFR, TNF-R-II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2
Protein Accession Number:
P20333
Protein Gi Number:
21264534
Ncbi Gene Id Number:
7133
User Note:
Centrifuge vial prior to opening.
Background:
TNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-123

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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