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To detect hPlGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human PlGF (38-152) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hPlGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human PlGF (38-152) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.

PlGF Antibody

CATALOG NUMBER: 38-151

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPlGF. Human PlGF specific antibody was purified by affinity chromatography employing immobilized hPlGF matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Direct:
To detect hPlGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Sandwich
To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PlGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Western Blot:
To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
PlGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PGF
Additional Names:
PGFL, PLGF, PlGF-2, D12S1900, SHGC-10760, PGFL, Placenta growth factorPlGF
Protein Accession Number:
P49763
Protein Gi Number:
17380553
Ncbi Gene Id Number:
5228
User Note:
Centrifuge vial prior to opening.
Background:
PLGF (also called Placenta Growth Factor) is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-151

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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