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To detect hGalectin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Galectin-3 (38-170) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGalectin-3.
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hGalectin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Galectin-3 (38-170) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGalectin-3.

Galectin-3 Antibody (biotin)

CATALOG NUMBER: 38-171

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Galectin-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.
Western Blot:
To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Galectin-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
LGALS3
Additional Names:
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3
Protein Accession Number:
P17931
Protein Gi Number:
215274262
Ncbi Gene Id Number:
3958
User Note:
Centrifuge vial prior to opening.
Background:
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-171

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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