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To detect hWNT-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human WNT-1 (38-176) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hWNT-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human WNT-1 (38-176) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.

WNT-1 Antibody

CATALOG NUMBER: 38-175

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1. Human WNT-1 specific antibody was purified by affinity chromatography employing immobilized hWNT-1 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
Sandwich
To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
Western Blot:
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
WNT-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
WNT1
Additional Names:
INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog,
Protein Accession Number:
P04628
Protein Gi Number:
139743
Ncbi Gene Id Number:
7471
User Note:
Centrifuge vial prior to opening.
Background:
Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta catenine. Mature human Wnt1 is a glycosylated protein containing 343 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-175

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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