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To detect mSF-20 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine SF-20 (38-190) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mSF-20 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine SF-20 (38-190) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.

SF-20 Antibody

CATALOG NUMBER: 38-189

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography employing immobilized mSF-20 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect mSF-20 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
Sandwich
To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
SF-20 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
D17Wsu104e
Additional Names:
Il25, Ly6elg, Il25, UPF0556 protein C19orf10 homolog, Interleukin-25, IL-25
Protein Accession Number:
Q9CPT4
Protein Gi Number:
61221740
Ncbi Gene Id Number:
28106
User Note:
Centrifuge vial prior to opening.
Background:
SF20, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-189

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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