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To detect Rat EGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat EGF (38-210) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF.
To detect Rat EGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat EGF (38-210) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF.

EGF Antibody (biotin)

CATALOG NUMBER: 38-211

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Rat EGF specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat EGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Western Blot:
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
AntiEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Egf
Additional Names:
Pro-epidermal growth factorEGF
Protein Accession Number:
P07522
Protein Gi Number:
1352360
Ncbi Gene Id Number:
25313
User Note:
Centrifuge vial prior to opening.
Background:
Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor is also a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-211

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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