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To detect hTIMP-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TIMP-1 (38-216) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.1ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved with a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.1ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved with a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.1ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved with a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
To detect hTIMP-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TIMP-1 (38-216) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.

TIMP-1 Antibody

CATALOG NUMBER: 38-215

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Human TIMP-1 specific antibody was purified by affinity chromatography employing immobilized hTIMP-1 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human TIMP-1 (3.0 μg/mL), a concentration of 5.0 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect hTIMP-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Sandwich
To detect hTIMP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human TIMP-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Western Blot:
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TIMP-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TIMP1
Additional Names:
EPA, EPO, HCI, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA
Protein Accession Number:
P01033
Protein Gi Number:
135850
Ncbi Gene Id Number:
7076
User Note:
Centrifuge vial prior to opening.
Background:
Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/mL) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-215

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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