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To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIP-1-alpha (38-222) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-alpha.
To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIP-1-alpha (38-222) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-alpha.

MIP-1a Antibody

CATALOG NUMBER: 38-220

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (human MIP-1 alpha). Human MIP-1a specific antibody was purified by affinity chromatography employing immobilized hMIP-1a matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hMIP-1a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.
Sandwich
To detect hMIP-1a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human MIP-1a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.
Western Blot:
To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL3
Additional Names:
MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha
Protein Accession Number:
P10147
Protein Gi Number:
127078
Ncbi Gene Id Number:
6348
User Note:
Centrifuge vial prior to opening.
Background:
MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-220

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

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