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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.

Resistin Antibody [1.2_3D7-1D11]

CATALOG NUMBER: 38-240

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Resistin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL.
Western Blot:
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
Purification:
This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Resistin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
RETN
Additional Names:
Adipocyte Secreted Factor, ADSF, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, Resistin, Adipose tissue-specific secretory factor, XCP1, RETN1
Protein Accession Number:
Q9HD89
Protein Gi Number:
18202962
Ncbi Gene Id Number:
56729
User Note:
Centrifuge vial prior to opening.
Background:
Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-240

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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