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To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Maspin (38-244) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMaspin.
To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Maspin (38-244) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMaspin.

Maspin Antibody (biotin)

CATALOG NUMBER: 38-278

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Human Maspin specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Human IL-17D by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IL-17D (38-266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D.
Western Blot:
To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
SERPINB5
Additional Names:
PI5, maspin, PI5, Serpin B5, Maspin, PI-5
Protein Accession Number:
P36952
Protein Gi Number:
229462757
Ncbi Gene Id Number:
5268
User Note:
Centrifuge vial prior to opening.
Background:
Maspin was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines. While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor. It plays an essential role in embryonic development through critical roles in cell adhesion. While the precise mechanism of maspin signaling is unclear, the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis and angiogenesis, while promoting apoptosis. Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-278

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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