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To detect hBAFF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BAFF (XP-5105Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBAFF.
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hBAFF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BAFF (XP-5105Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBAFF.

BAFF Antibody

CATALOG NUMBER: XP-5105

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBAFF (human BAFF).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hBAFF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBAFF.
Western Blot:
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hBAFF specific antibody was purified by affinity chromatography employing immobilized hBAFF matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
BAFF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNFSF13B
Additional Names:
DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS
Protein Accession Number:
Q9Y275
Protein Gi Number:
13124573
Ncbi Gene Id Number:
10673
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5105

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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