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To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat CNTF (XP-5114) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF.
To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat CNTF (XP-5114) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF.

CNTF Antibody (biotin)

CATALOG NUMBER: XP-5114Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat CNTF (rat CNTF).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat CNTF (XP-5114) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.
Western Blot:
To detect rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-rat CNTF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
CNTF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Cntf
Additional Names:
Ciliary neurotrophic factorCNTF
Protein Accession Number:
P20294
Protein Gi Number:
116587
Ncbi Gene Id Number:
25707
User Note:
Centrifuge vial prior to opening.
Background:
Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5114Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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