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To detect hCNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCNTF.
To detect hCNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCNTF.

CNTF Antibody (biotin)

CATALOG NUMBER: XP-5115Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCNTF (human CNTF).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich: To detect hCNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCNTF.
Western Blot:
To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hCNTF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
CNTF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CNTF
Additional Names:
HCNTFCiliary neurotrophic factorCNTF
Protein Accession Number:
P26441
Protein Gi Number:
116585
Ncbi Gene Id Number:
1270
User Note:
Centrifuge vial prior to opening.
Background:
Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5115Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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