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IL-6 Antibody (biotin)

CATALOG NUMBER: XP-5195Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-6 (rat IL-6).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Rat IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-6 (XP-5195) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.
Western Blot:
To detect rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-rat IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-6 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il6
Additional Names:
ILg6, Ifnb2, Il-6, Interleukin-6IL-6
Protein Accession Number:
P20607
Protein Gi Number:
124350
Ncbi Gene Id Number:
24498
User Note:
Centrifuge vial prior to opening.
Background:
IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5195Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
Shipping Info

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