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To detect hTACI by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TACI (XP-5278Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTACI.
To detect hTACI by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TACI (XP-5278Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTACI.

TACI Antibody

CATALOG NUMBER: XP-5278

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human TACI.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect human TACI by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human TACI.
Sandwich:
To detect hTACI by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TACI (XP-5278Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTACI.
Western Blot:
To detect human TACI by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TACI is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 1215 - U937 Cell Lysate
Purification:
Anti-hTACI specific antibody was purified by affinity chromatography employing immobilized hTACI matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TACI antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNFRSF13B
Additional Names:
CVID, RYZN, TACI, CD267, CVID2, TNFRSF14B
Protein Accession Number:
Q4ACX1
Protein Gi Number:
121943629
Ncbi Gene Id Number:
23495
User Note:
Centrifuge vial prior to opening.
Background:
TACI is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein binds to both APRIL and BAFF and induces activation of the transcription factors NFAT (nuclear-factor of activated T cells), AP1, and NF kappa B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith Magenis syndrome region on chromosome 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5278

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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