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To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human TECK (XP-5280) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTECK.
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human TECK (XP-5280) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTECK.

TECK Antibody (biotin)

CATALOG NUMBER: XP-5280Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (Human TECK).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TECK (XP-5280) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.
Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hTECK specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TECK antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL25
Additional Names:
TECK, Ckb15, SCYA25, TECK, C-C motif chemokine 25, Chemokine TECK,
Protein Accession Number:
O15444
Protein Gi Number:
212286066
Ncbi Gene Id Number:
6370
User Note:
Centrifuge vial prior to opening.
Background:
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5280Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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